Influence of Exogenous Enzymes on Endogenous Digestive ...

致谢 · 中文摘要 · 引言 · 文献综述 · 1. 家禽消化生理特点 · 1.1 各主要消化器官 · 1.2 家禽消化道酸度及几种主要消化酶 · 2. 外源酶对家禽内源消化酶的影响 ... Contents 致谢中文摘要引言文献综述    1.家禽消化生理特点        1.1各主要消化器官        1.2家禽消化道酸度及几种主要消化酶    2.外源酶对家禽内源消化酶的影响        2.1酶活的研究方法        2.2外源酶对家禽内源消化酶活性的影响    3.外源酶对家禽饲料氨基酸消化率的影响        3.1家禽饲料氨基酸消化率的研究方法        3.2外源酶对家禽饲料蛋白质及氨基酸消化率的影响研究内容    1.材料与方法        1.1试鸡的选择与饲养管理        1.2试鸡日粮        1.3试验设计与试验方法        1.4样品的采集与处理        1.5测定指标        1.6数据统计与分析方法    2.结果与分析        2.1胃肠道及胰腺组织中各种酶的活性        2.2预试并强饲加酶饲料及预试期对鸡饲料氨基酸消化率的影响    3.讨论        3.1胃肠道及胰腺组织的各种酶活        3.2预试日粮、强饲饲料加酶与否及预试期对鸡饲料氨基酸消化率的影响    4.结论参考文献英文摘要 InfluenceofExogenousEnzymesonEndogenousDigestiveEnzymeActivityandFeedAminoAcidDigestibilityofPoultryCN 姚建国 南京农业大学 Abstract：80eighteen-week-oldISABrowncockerelswererandomlyallocatedtosixgroups.Fivecockerelswereusedtodetermineendogenousaminoacidexcrementalamountasblankexperiment.Therewerefifteencockerelsineachgroupofotherfivegroups.FivecockerelswereusedtodetermineAAD.Theothertencockerelswereusedforcollectingsamplestomeasureendogenousdigestiveenzymeactivities,fiveofthemwereslaughteredtocollectsamplesattheendofpre-experimentperiod,andtheotherswereslaughteredafterfastingfor4h.Cockerelsofthefirstexperimentalgroupwerefedbycommondietwithoutenzymeandtheotherfourgroup抯werefeddietswithenzyme.Inthefourgroups,fivebirdswerefeddietwithenzymeforonetofourweekpre-experimentalperiod,respectivelyforadifferentpre-experimentalperiodstrial,thenfeaceswerecollectedtodetermineAAD.Allofthesegroups,theAADweredeterminedby揟ME?method.Theresultsshowed:（1）Proventriculuspepsinactivitiesofcockerelswereincreasedbutnotsignificantlywhenbirdswerefedbythedietwithenzymefordifferentpre-experimentalperiod.Pepsinactivitieswereincreasedbyfasting.（2）Therewasnosignificanteffectorroleontrypsin,amylaseactivitiesofduodenum,jejunumandpancreasbyenzymesupplementation.Trypsinandaniylaseactivitiesofedistalduodenumwerehigherorsignificanthigherthanthatofproximalduodenum.Trypsinspecificactivitiesofdistalduodenum,jejunumandpancreaswereincreased,butnoeffectontheproximalduodenumbyfasting.Theaniylaseactivitiesofduodenumandjejunumwerereducedsignificantlybyfasting.（3）Cellulaseactivitiesofdigestivetractchymewereincreasedsignificantlybyexogenousenzyme,butalsoreducedsignificantlybyfasting.Cellulaseactivitiesofproventriculusweresignificanthigherthanthatofduodenumandjejunum.（4）Apparentdigestibilityandtruedigestibilityofseventeenaminoacidwereincreasedindifferentamountfor54differentaminoacidbyexogenousenzymeandvariouspre-experimentalperiod.ThedigestibilitiesofMet,His,Thr,Lys,Ser,GlyandAlaoftherationwithexogenousenzymewerehigherorveryhigherthanofcommondietgroup（p