Difference Between CFSE and LFSE

The term LFSE stands for ligand field stabilization energy which is a term in the Ligand field theory. It describes bonding, orbital arrangement ... Home/Science&Nature/Science/Chemistry/AnalyticalChemistry/DifferenceBetweenCFSEandLFSE ThekeydifferencebetweenCFSEandLFSEisthatthetermCFSEstandsforachemicalcompound,whereasthetermLFSEstandsforatheoryinchemistry. ThetermCFSEstandsforthechemicalcompoundcarboxyfluoresceinsuccinimidylester.Itisadyethatwemainlyuseforstainingcellsforfluorescence.ThetermLFSE,ontheotherhand,standsforligandfieldstabilizationenergy,whichisatermintheLigandfieldtheory. CONTENTS 1.OverviewandKeyDifference 2.WhatisCFSE  3.Whatis LFSE 4.SidebySideComparison–CFSEvsLFSEinTabularForm 5.Summary WhatisCFSE? ThetermCFSEstandsforthechemicalcompoundcarboxyfluoresceinsuccinimidylester.Itisusefulasafluorescentcellstainingdye.Thissubstanceiscell-permeableandcovalentlycoupleswithintracellularmoleculesviathesuccinimidylgroupofCFSE.Notably,thisoccurswithintracellularlysineresiduesandotheraminesources.DuetothiscouplingreactionbetweenCFSEmoleculesandintracellularmolecules,thisfluorescentdyeisretainedwithincellsforextremelylongperiods. ThechemicalformulaofCFSEmoleculeisC25H15NO9.Themolarmassis473g/mol.Often,CFSEgetsconfusedwithCFDA-SE(carboxyfluoresceindiacetatesuccinimidylester)duetotheirclosesimilarity.Buttheyarenotthesamemolecule,andCFSEishighlycell-permeablecomparedtoCFDA-SE.Also,thissecondcompoundisnon-fluorescent. Originally,theCFSEcompoundwasproducedasafluorescentdyethatcanstablylabellymphocytesandtracktheirmigrationthroughbloodwithinanimalsforalongtime.Subsequently,itwasrevealedthatthedyecanbeusedtomonitorlymphocyteproliferation.However,ifweuseahighconcentrationwithinthecells,itistoxictotheanimal. WhatisLFSE? ThetermLFSEstandsforligandfieldstabilizationenergywhichisatermintheLigandfieldtheory.Itdescribesbonding,orbitalarrangementandothercharacteristicsofcoordinationcomplexes.Thistheoryrepresentsanapplicationofmolecularorbitaltheorytotransitionmetalcomplexes.Usually,atransitionmetalhasninevalenceatomicorbitals.Therearefivedorbitals,onesorbitalandthreeorbitalsthatcanbetakenasvalenceshells.Theseorbitalscontaintheappropriateenergytoformachemicalbondwithaligand.Thisamountofenergyiscalledtheligandfieldstabilizationenergy.Moreover,thischemicalconceptisusuallyappliedaccordingtothegeometryofthecomplex.Mostly,itexplainsabouttheoctahedralcomplexescontainingsixligandspermolecule. WhatistheDifferenceBetweenCFSEandLFSE? ThetermCFSEstandsforthechemicalcompoundcarboxyfluoresceinsuccinimidylesterwhilethetermLFSEstandsforligandfieldstabilizationenergywhichisatermintheLigandfieldtheory.Therefore,thekeydifferencebetweenCFSEandLFSEisthatthetermCFSEstandsforachemicalcompound,whereasthetermLFSEstandsforatheoryinchemistry.Tobemorespecific,CFSEisafluorescentdyewhileLFSEisthe energyrequiredtostablyformacoordinationcomplex. Thefollowinginfographicsummarizesthe differencebetweenCFSEandLFSEintabularform. Summary– CFSEvsLFSE ThetermCFSEstandsforthechemicalcompoundcarboxyfluoresceinsuccinimidylesterwhilethetermLFSEstandsforligandfieldstabilizationenergywhichisatermintheLigandfieldtheory.Therefore,thekeydifferencebetweenCFSEandLFSEisthatthetermCFSEstandsforachemicalcompound,whereasthetermLFSEstandsforatheoryinchemistry. Reference: 1.“CarboxyfluoresceinSuccinimidylEster.”Wikipedia,WikimediaFoundation,5June2020,Availablehere. 2.“LigandFieldTheory.”Wikipedia,WikimediaFoundation,16June2020,Availablehere. ImageCourtesy: 1.“Carboxyfluoresceinsuccinimidylester”  ByEdgar181(talk)–Ownwork(PublicDomain)viaCommonsWikimedia Relatedposts: DifferenceBetweenCriticalPointandTriplePoint DifferenceBetweenSingleBeamandDoubleBeamSpectrophotometer DifferenceBetweenMicroAnalysisandSemiMicroAnalysis DifferenceBetweenProximateandUltimateAnalysisofCoal DifferenceBetweenAcidValueandSaponificationValue AbouttheAuthor:MadhuMadhuisagraduateinBiologicalScienceswithBSc(Honours)DegreeandcurrentlypersuingaMastersDegreeinIndustrialandEnvironmentalChemistry.Withamindrootedfirmlytobasicprincipalsofchemistryandpassionforeverevolvingfieldofindustrialchemistry,sheiskeenlyinterestedtobeatruecompanionforthosewhoseekknowledgeinthesubjectofchemistry. LeaveaReplyCancelreply Youremailaddresswillnotbepublished.Requiredfieldsaremarked*CommentName* Email* Website FeaturedPosts YouMayLike LatestPosts WhatistheDifferenceBetweenHeparSulphandSilicea WhatistheDifferenceBetweenHollidayModelandMeselson-RaddingModel WhatistheDifferenceBetweenResearchProblemandResearchQuestion WhatistheDifferenceBetween12Additionand14Addition WhatistheDifferenceBetweenNeurodevelopmentalandNeurocognitiveDisorders WhatistheDifferenceBetweenMethylcobalaminandAdenosylcobalamin Home Vacancies About RequestArticle ContactUs