基因體DNA萃取

Learn about methods for genomic DNA extraction, isolation, and purification. Find genomic DNA isolation kits. PopularApplications&TechniquesShopAllProductsServicesSupport Signin QuickOrder     Search ThermoFisherScientific SearchAll Search Home›LifeSciences›NucleicAcidExtractionandPurification›GenomicDNAExtractionandGenomicDNAIsolationGenomicDNAExtractionandGenomicDNAIsolationSeeNavigation‹NucleicAcidExtractionandPurificationAutomatedExtractionforDNA,RNA,Protein&Cells ›GenomicDNAExtractionandGenomicDNAIsolation IsolationofDNAFragmentsandPCRClean-Up GenomicDNAExtractionbySampleType DNAzolReagentFamilyforGenomicDNAIsolation GeneCatcherMagneticBeads PlasmidDNAIsolation ›RNAExtractionKitsandRNAIsolationReagents ›ViralNucleicAcidIsolationKits ›RNA/DNAQuantification ›NucleicAcidGelElectrophoresis ›NucleicAcidLabeling ›DNA&RNAPurification&AnalysisEquipment&Plasticware ›NucleicAcidIsolationProtocolVideos ›NucleicAcidIsolationBrochures,ManualsandGuides ›NucleicAcidIsolationApplicationNotes ›GenomicDNAisolationandgenomicDNAextractionarerelatedtermsthatrefertogenerationofpurifiedgenomicDNAfromagivenstartingmaterialsuchascellsortissue.Let’stalkabouteachterminabitmoredetail.  WhatisgenomicDNAextraction? GenomicDNAextractionistheprocessofreleasingchromosomalDNAfromthecellularmatrixinwhichitiscontained.GenomicDNAextractionrequiresarobustdisruptionmethodtoopenthenucleiandcellwalls(ifapplicable);itusuallyinvolvesaddingacompatibledetergentaswellasmechanicalshearing.FollowinggenomicDNAextractionisisolation,inwhichtheproteins,cellmembranes,andothercellularmaterialsareremovedyieldingapurifiedgenomicDNAsample.  MethodsforgenomicDNAisolation ThreegeneralmethodsforgenomicDNAisolationarecommon,andeachisbasedonadifferentbiochemicalprinciple.Selectionofamethodisbasedonthethroughputrequired,equipmentavailableinthelab,andhowthepurifiedgenomicDNAwillbeused.  Organicextractionandprecipitation GenomicDNAisolationbyorganicextractioninvolvestheadditionofphenolandquanidineisothiocynatetoseparatetheDNAandproteinsintodifferentorganicphases.Organicextractionisalow-costmethodand,withadvancedreagentssuchasDNAzol,isastraightforwardmethodrequiringverylittleequipment.  Silica DNAbindstosilica(akaglassfibers)underhigh-saltconditionsandcanbereleasedunderlow-saltconditions.Silica-containingcolumnsprovideaneasywaytobind,wash,andelutepurifiedgenomicDNAfrommultipleclarifiedcelllysatesinparallel.Columnsaredesignedtoflowbuffersthroughcentrifugation,vacuum,orgravity.PureLinkGenomicDNAMiniKits usespincolumntechnologyandworkinamicrocentrifugeforeasyprepofupto18samplesatatime.Spinplatesprovidethesameisolationtechnologyinahigh-throughput,automation-friendlyformat.  Paramagneticbeads Inthismethod,paramagnetic(attractedtomagnet)beadsareaddedtothesample,andgenomicDNAbindstothebeads.Usingastrongmagnet,thebeadsareheldinplacewhileremovingunwantedmaterial.Afterwashing,thegenomicDNAiselutedfromthebeadsinwateroralow-saltbuffer.Thebead-basedmethod,usedinMagMAXmulti-sampleDNAisolation andrelatedkits,isscalableandautomationcompatible.  SimplifyyourgenomicDNAextractionprocesswithThermoFisherScientifickitsandreagents GenomicDNAextractionkitscontainallofthereagentsneededtogetyoufromsampletopurifiedDNA.ThermoFisherScientificoffersGenomicDNAextractionkitsinyourchoiceofformatsforspecificsampletypes:TissueDNAextractionkits BloodDNAextractionkits CellDNAextractionkits PlantDNAextractionkitsForensicDNAextractionkits BiopharmaceuticalDNAextractionkits BacterialDNAextractionkits BuccalDNAextractionkits ResourcesforgenomicDNAisolation NucleicAcidPurificationandAnalysisSupportCenterFindtips,troubleshootinghelp,andresourcesforyournucleicacidpurification&analysisapplications.   Protocol:PurificationofgenomicDNAusingPurelinkSilica  DNAPurificationandAnalysisGuide  KingFisherInstrumentsGuide  DynabeadsMagneticBeadsGuide   Seeallnucleicacidisolationbrochures,manualsandguidesForResearchUseOnly.Notforuseindiagnosticprocedures. 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