MOPS Buffer (10X) (0.2 M, pH 7) Preparation and Recipe
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MOPS Buffer (10X) (0.2 M, pH 7) Preparation and Recipe · Prepare 800 mL of dH2O in a suitable container. · Add 41.86 g of MOPS free acid to the solution. · Add 4.1 ... Activelyhelpingcustomers,employeesandtheglobalcommunityduringthecoronavirusSARS-CoV-2outbreak. Learnmore>>XNavigationMenuHomeSignInShoppingCartOrderingInformationProductsServicesToolsResourcesAboutUsCareersContactUsDistributorsTermsofUseTermsofSalesPrivacyAATBioquest MOPSBuffer(10X)(0.2M,pH7)PreparationandRecipeMOPSbufferisoftenusedinpolyacrylamidegelelectrophoresis.Toprepare Lof MOPSBuffer(10X)(0.2M,pH7):ChangethevalueinthetextboxabovetoscaletherecipevolumeTable1.RequiredcomponentsComponentAmountConcentrationMOPSfreeacid(mw:209.26g/mol)41.86g0.2MSodiumAcetate(mw:82.03g/mol)4.1g0.05MDisodiumEDTA(mw:372.24g/mol)3.72g0.01MPrepare800mLofdH2Oinasuitablecontainer.Add41.86gofMOPSfreeacidtothesolution.Add4.1gofSodiumAcetatetothesolution.Add3.72gofDisodiumEDTAtothesolution.AdjustsolutiontodesiredpHusingNaOH(typicalpH=7)AdddH2Ountilthevolumeis1L.Tomakeapurchaseinquiryforthisbuffer,pleaseprovideyouremailaddressbelow:RequestquotationPhysiologicalBufferpHBufferingSamplePreparationBioAssaysMiscCell/Culture/GrowthMediaGelElectrophoresisName▲▼PBS(PhosphateBufferedSaline)(1X,pH7.4)HBSS(Hank'sBalancedSaltSolution)HHBS(Hank'sBufferwithHepes)HEPESBuffer(1M,7.5pH)PBSwithCa2+andMg2+(D-PBS)ExtracellularSaline(1x,pH7.3)Tyrode'sSolution,AcidicName▲▼CitrateBuffer(pH3.0to6.2)AcetateBuffer(pH3.6to5.6)TrisBuffer(1M,pH7.2)CitrateBuffer(0.1M,pH6.0)AcetateBuffer(0.1M,pH5.0)PhosphateBuffer(pH5.8to7.4)HydrochloricAcid-PotassiumChlorideBuffer(0.1M,pH2.0)Glycine-HClBuffer(0.1M,pH3.0)Citrate-PhosphateBuffer(0.15M,pH5.0)Glycine-SodiumHydroxideBuffer(0.08M,pH10)EBSS(magnesium,calcium,phenolred)(pH7.0)Imidazole-HClBuffer(0.05M,pH7.0)Trizma®Buffer(pH7.0to9.2)MES(0.5M,pH6)Bis-TrisBuffer(1M,6.5pH)ADABuffer(0.5M,6.6pH)ACESBuffer(0.1M,6.7pH)PIPESBuffer(1M,6.8pH)MOPSOBuffer(0.5M,6.9pH)BES-BufferedSaline(2X)(0.05M,pH6.95)TESBuffer(1M,7.5pH)MOPSBuffer(10X)(0.2M,pH7)SSCbuffer(20X)(3M,pH7)TBS(1M,pH7.4)Bicine(1M,pH8.26)Tricine(1M,pH8.05)TAE(1M,pH8.6)CAPS(0.5M,pH10.4)HEPPSO(1M,pH7.85)CHES(0.5M,pH9.5)PotassiumPhosphate(pH5.8to8.0)Diethanolamine(1M,pH9.8)SodiumBorateBuffer(1M,pH8.5)MaleicAcid(1M,pH7.5)Tris-BufferedSaline(TBS,0.1M)AmmoniumSulfate,SaturatedPotassiumPhosphateBuffer(1M,pH6.5)Citrate-PhosphateBuffer(50mM,pH5.6)Citrate-PhosphateBuffer(20mM,pH5.6)SodiumPhosphateSolutionSodiumAcetate(3M,pH5.2)TBBufferTris-citrateBuffer(pH8.0)BorateBufferGlycine(0.1M,pH2.2)AmmoniumBicarbonate(50mM,pH7.8)AmmoniumAcetateCarbonate-BicarbonateBuffer(pH9.2to10.6)EntireTableNameItemsperpage:510All1-10of48⟨⟩Name▲▼TEBuffer10XElutionBufferBACDNAMicroinjectionBufferEDTASolutionACKLysisBufferParaformaldehydeSolution(8%)Gey'sBalancedSaltSolutionLysisSolutionH-50BufferAlkalineSodiumThiosulfateOsmiumTetroxideSolutionDEAE-dextranTrichloroaceticAcid(TCA)EntireTableNameItemsperpage:510All1-10of13⟨⟩Name▲▼BradfordStockSolutionAnesthetic(MS222)Solution5-Fluorocytosine(5-FC)SolutionSSC(NorthernBlot,20x)SSPE(Blot,20x,pH7.4)ImmunofluorescenceQuenchingSolutionNuclearFastRedSolutionPyroninY(PY)SolutionMayer'sHemalumChloroquineEthidiumBromideSolutionCresolRedSolutionEntireTableNameItemsperpage:510All1-10of12⟨⟩Name▲▼SodiumIodide/SodiumSulfiteSolutionDithiothreitol(DTT)(1M)Name▲▼BathSolution(Ca2+/Mg2+Free)ModifiedBarth'sSaline(MBS)CalciumChlorideStockSolutionYeastTwoHybrid(Y2H)Media,AminoAcidDropoutMixesDigestionMediumChelationMediumPenicillin/Streptomycin/ChloramphenicolAntibioticMixEmbryonicMediumCarbenicillinSolutionM9MinimalSaltsIPTGSolutionEntireTableNameItemsperpage:510All1-10of11⟨⟩Name▲▼SDS-PAGESDSRunningBuffer(10x)ElectrophoreticTransferBuffer(20x)Tris-GlycineTransferBuffer(20x)SodiumCarbonateTransferBuffer(40x,pH9.5)AcrylamideStockSolutionImidazole-SDSSolutionElectrodeBufferTris/EACABufferReferencesThisonlinetoolmaybecitedasfollowsMLA"Quest Calculate™ MOPSBuffer(10X)(0.2M,pH7)PreparationandRecipe." AATBioquest,Inc., 15 Sep. 2022, https://www.aatbio.com/resources/buffer-preparations-and-recipes/mops-buffer-10x-ph-7.APAAATBioquest,Inc.(2022, September 15). Quest Calculate™ MOPSBuffer(10X)(0.2M,pH7)PreparationandRecipe.AATBioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/mops-buffer-10x-ph-7.Location:Home/BufferPreparationandRecipes/MOPSBuffer(10X)(0.2M,pH7)PreparationandRecipeAboutPrivacyTermsofUseTermsofSalesDistributorsCopyright©2021AATBioquest,Inc.AllRightsReserved.
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